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Wei sheng wu xue bao = Acta microbiologica Sinica

[Construction of ectoine absorption defective mutant for efficient ectoine production].


PMID 22803353

Abstract

To further enhance ectoine (1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) synthesis efficiency. We cloned Halomonas salina DSM 5928(T) teaABC gene of ectoine-specific transporter TeaABC by walking PCR and constructed ectoine absorption defective mutant H. salina DSM 5928(T) (teaABC-) by Red recombination technology. The total concentration of ectoine and productivity in a 10 L fermentor were 9.10 (+/- 0.08) g/L and 9.93 (+/- 0.09) g/L. d. The ectoine absorption defect mutant H. salina DSM 5928(T) (teaABC-) compromised the negative feedback regulation of ectoine synthesis, which can significantly improve the efficiency of ectoine production.

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