EMAIL THIS PAGE TO A FRIEND

Biomacromolecules

Mesenchymal stem cell interactions with 3D ECM modules fabricated via multiphoton excited photochemistry.


PMID 22876971

Abstract

To understand complex micro/nanoscale ECM stem cell interactions, reproducible in vitro models are needed that can strictly recapitulate the relative content and spatial arrangement of native tissue. Additionally, whole ECM proteins are required to most accurately reflect native binding dynamics. To address this need, we use multiphoton excited photochemistry to create 3D whole protein constructs or "modules" to study how the ECM governs stem cell migration. The constructs were created from mixtures of BSA/laminin (LN) and BSA alone, whose comparison afforded studying how the migration dynamics are governed from the combination of morphological and ECM cues. We found that mesenchymal stem cells interacted for significantly longer durations with the BSA/LN constructs than pure BSA, pointing to the importance of binding cues of the LN. Critical to this work was the development of an automated system with feedback based on fluorescence imaging to provide quality control when synthesizing multiple identical constructs.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

S3388
Sulforhodamine 101 acid chloride, Technical grade
C31H29ClN2O6S2