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Chemical communications (Cambridge, England)

Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry.


PMID 23032097

Abstract

Toward new biotechnology by genetic alphabet expansion, we developed an efficient site-specific labeling method for large RNA molecules. The combination of unnatural base pair transcription and post-transcriptional modification by click chemistry enables simple RNA labeling with a wide variety of functional groups at desired positions, in a one-pot reaction.

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