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Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology

[Effect of AKR7A1 overexpression on crotonaldehyde-induced mutagenicity in V79-4 cells].


PMID 23046928

Abstract

To investigate the effect of overexpressing rat aldo-keto reductases (AKR7A1) in V79-4 cells on crotonaldehyde-induced mutagenicity. The expression level and enzyme activity of AKR7A1 protein expressed in V79-4 cells were measured by Western blotting and AKR enzyme assay, respectively. HGPRT assay was applied to evaluate the effect of AKR7A1 overexpression on crotonaldehyde-induced mutagenicity in V79-4 cells. Western blotting confirmed a high expression level of AKR7A1 protein in V79-4 cells. AKR enzyme assay showed that the overexpressed AKR7A1 protein was functionally active. HGPRT assay demonstrated that cells overexpressing AKR7A1 exhibited a significant increase in resistance to crotonaldehyde-induced mutagenicity compared to control cells. Overexpression of AKR7A1 can protect V79-4 cells against crotonaldehyde-induced mutagenicity.