EMAIL THIS PAGE TO A FRIEND

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

The analytical application and spectral investigation of DNA-CPB-emodin and sensitive determination of DNA by resonance Rayleigh light scattering technique.


PMID 23103464

Abstract

A new sensitive DNA probe containing cetylpyridinium bromide (CPB) and emodin (an effective component of Chinese herbal medicine) was developed using the resonance Rayleigh light scattering (RLS) technique. A novel assay was first developed to detect DNA at nanogram level based on the ternary system of DNA-CPB-emodin. The RLS signal of DNA was enhanced remarkably in the presence of emodin-CPB, and the enhanced RLS intensity at 340.0 nm was in direct proportion to DNA concentration in the range of 0.01-2.72 μg mL(-1) with a good linear relationship. The detection limit was 1.5 ng mL(-1). Three synthetic DNA samples were measured obtaining satisfactory results, the recovery was 97.6-107.3%.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

30269
Emodin, analytical standard
C15H10O5