EMAIL THIS PAGE TO A FRIEND

Lab on a chip

Passive circulating cell sorting by deformability using a microfluidic gradual filter.


PMID 23147069

Abstract

The deformability of circulating leukocytes plays an important role in the physiopathology of several diseases like sepsis or acute respiratory distress syndrome (ARDS). We present here a microfluidic method for the passive testing, sorting and separating of non-adherent cell populations by deformability. It consists of microfluidic sieves in series with pore sizes decreasing from the upstream to the downstream. The method capabilities are demonstrated with monocytic cell lines (THP-1) treated by Jasplakinolide (a stabilizer of polymerized actin), LatrunculinA (an inhibitor of actin polymerization), and with the plasma of patients suffering from ARDS. Simple sample injection with standard syringes and pumps makes the method readily adapted for experimentation in hospitals.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

J4580
Jasplakinolide, ≥97% (HPLC)
C36H45BrN4O6