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Bioscience, biotechnology, and biochemistry

Cloning, heterologous expression, and enzymatic characterization of cathepsin L from starfish (Asterina pectinifera).


PMID 23221686

Abstract

To determine the role of cathepsin L in Echinoderms, starfish (Asterina pectinifera) cathepsin L (ApCtL) was cloned. The results of RT-PCR analysis indicated that the expression of ApCtL in all of the tissues. The pro-mature enzyme of ApCtL, proApCtL, was expressed in Escherichia coli, and cathepsin activity was detected by cleaving of synthetic fluorogenic peptide substrates and gelatin zymography.

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C6854 Cathepsin L from human liver, ≥0.5 units/mg protein, solution