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Biosensors & bioelectronics

One-step synthesis of graphene oxide-thionine-Au nanocomposites and its application for electrochemical immunosensing.


PMID 23584387

Abstract

Here we report a one-step approach to synthesize graphene oxide-thionine-Au (GO-Thi-Au) nanocomposites using the synergistic effect of Thi and GO. Thi molecules adsorbed on the nanocomposites still kept the property of electroactive redox. Meanwhile, the resulting Au nanoparticles (AuNPs) of the nanocomposites offer active sites for the immobilization of biomolecules. The GO-Thi-Au nanocomposites are successfully employed for the fabrication of a simple, label-free, and ultrasensitive electrochemical immunosensor. Using carcinoembryonic antibody (anti-CEA) and carcinoembryonic antigen as model systems, the immunosensor showed an ultralow detection limit of 0.05fgmL(-1), and a linear calibration plot was obtained in a wide concentration range from 0.1fgmL(-1) to 1×10(9)fgmL(-1).