EMAIL THIS PAGE TO A FRIEND

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

[Effect of cordyceps polysaccharide on lipid peroxidation of rats with dimethylnitrosamine-induced liver fibrosis].


PMID 23668016

Abstract

To observe the pharmacological effect of Cordyceps polysaccharide on dimethylnitrosamine (DMN)-induced liver fibrosis in rats. DMN rat liver fibrosis model was established and divided into the normal group (N, n = 6), the model group (M, n = 11), the Cordyceps polysaccharide group (C, n = 8) and the colchicine group (Q, n = 9). During the modeling for four weeks, Cordyceps polysaccharide (60 mg x kg(-1)) and colchicine (0.1 mg x kg(-1)) were orally administered for three weeks, while the model and normal groups were given disinfected water of the same amount. serum ALT, AST, GGT and Alb, TBil content; content of hydroxyproline (Hyp) in liver tissues; liver pathology and collagen staining; SOD activity and MDA, GSH, GSH-Px in liver tissues; protein expression of proliferating cell nuclear antigen (PCNA) in liver tissues. Serum ALT, AST, GGT, TBil significantly increased, and A1b decreased significantly in the model group. Hepatic Hyp significantly increased in the model group, whereas the index remarkably decreased in the Cordyceps polysaccharide group and the colchicine group. HE staining: the structure of normal hepatic lobules was damaged, with hepatocytes tumefaction and proliferation of connective tissues in portal tracts in the model group, while the Cordyceps polysaccharide group and the colchicine group recorded notable reduction in above pathological changes. Collagen staining: the model group showed hepatic lobule fibrous septum and many intact pseudolobules; while the Cordyceps polysaccharide group and the colchicine group witnessed decrease in collagen deposition. The model group showed significant decrease in SOD, GSH-Px and GSH and increase in MDA, whereas the Cordyceps polysaccharide group and the colchicine group recorded notable growth in GSH and GSH-Px. The model group showed significant decrease in protein expression of PCNA in liver tissues, while the Cordyceps polysaccharide group and the colchicine group showed significant reduction. Cordyceps polysaccharide can significantly inhibit DMN-induced liver fibrosis and lipid peroxidation in rats.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

G9270 γ-Glutamyltranspeptidase from equine kidney, Type VI, 5-12 units/mg solid
G2262 γ-Glutamyltranspeptidase from porcine kidney, Type IV, 2.0-6.0 units/mg solid
G2751 Glutamic-Oxalacetic Transaminase from porcine heart, Type I, ammonium sulfate suspension, 200-500 units/mg protein
G8255 Glutamic-Pyruvic Transaminase from porcine heart, lyophilized powder, ≥75 units/mg protein
G9880 Glutamic-Pyruvic Transaminase from porcine heart, ammonium sulfate suspension, ≥75 units/mg protein
G6137 Glutathione Peroxidase from bovine erythrocytes, lyophilized powder, ≥300 units/mg protein
G4013 Glutathione Peroxidase from human erythrocytes, lyophilized powder, ≥30 units/mg protein
48552
N-Nitrosodimethylamine, analytical standard
C2H6N2O
48670 N-Nitrosodimethylamine solution, certified reference material, 200 μg/mL in methanol
S9697
Superoxide Dismutase bovine, recombinant, expressed in E. coli, lyophilized powder, ≥2500 units/mg protein, ≥90% (SDS-PAGE)
S5395
Superoxide Dismutase from bovine erythrocytes, BioReagent, ≥3,000 units/mg protein, suitable for cell culture, lyophilized powder
S7571
Superoxide Dismutase from bovine erythrocytes, lyophilized powder, ≥3,000 units/mg protein
S7446
Superoxide Dismutase from bovine erythrocytes, BioUltra, lyophilized powder, ≥4,500 units/mg protein, ≥97% (SDS-PAGE)
S8160
Superoxide Dismutase from bovine liver, lyophilized powder, ≥1500 units/mg protein
S8409
Superoxide Dismutase from bovine liver, ammonium sulfate suspension, 2,000-6,000 units/mg protein (biuret)
S5639
Superoxide Dismutase from Escherichia coli, lyophilized powder, ≥1,000 units/mg protein
S5389
Superoxide Dismutase from Escherichia coli, lyophilized powder, ≥1,000 units/mg protein
S9636
Superoxide Dismutase from human erythrocytes, essentially salt-free, lyophilized powder, ≥2,500 units/mg protein
S9076 Superoxide Dismutase I human, recombinant, expressed in E. coli
H5534
trans-4-Hydroxy-L-proline, BioReagent, suitable for cell culture, ≥98.5%
C5H9NO3
H54409
trans-4-Hydroxy-L-proline, ≥99%
C5H9NO3
41875
trans-4-Hydroxy-L-proline, analytical standard
C5H9NO3