EMAIL THIS PAGE TO A FRIEND

Nanotoxicology

Uptake and toxicity of copper oxide nanoparticles in cultured primary brain astrocytes.


PMID 23889294

Abstract

To test for consequences of an exposure of brain cells to copper oxide nanoparticles (CuO-NPs), we synthesised and characterised dimercaptosuccinate-coated CuO-NPs. These particles had a diameter of around 5 nm as determined by transmission electron microscopy, while their average hydrodynamic diameter in aqueous dispersion was 136 ± 4 nm. Dispersion in cell-culture medium containing 10% fetal calf serum increased the hydrodynamic diameter to 178 ± 12 nm and shifted the zeta potential of the particles from -49 ± 7 mV (in water) to -10 ± 3 mV. Exposure of cultured primary brain astrocytes to CuO-NPs increased the cellular copper levels and compromised the cell viability in a time-, concentration- and temperature-dependent manner. Application of CuO-NPs in concentrations above 100 µM copper (6.4 µg/ml) severely compromised the viability of the cells, as demonstrated by a lowered 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction capacity, a lowered cellular lactate dehydrogenase activity and an increased membrane permeability for the fluorescent dye propidium iodide. Copper internalisation as well as cell toxicity of astrocytes exposed to CuO-NPs were similar to that observed for cells that had been incubated with copper salts. The CuO-NP-induced toxicity was accompanied by an increase in the generation of reactive oxygen species (ROS) in the cells. Both, ROS formation and cell toxicity in CuO-NP-treated astrocytes, were lowered in the presence of the cell-permeable copper chelator tetrathiomolybdate. These data demonstrate that CuO-NPs are taken up by cultured astrocytes and suggest that excess of internalised CuO-NPs cause cell toxicity by accelerating the formation of ROS.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

544868
Copper(II) oxide, nanopowder, <50 nm particle size (TEM)
CuO
450812
Copper(II) oxide, powder, 99.99% trace metals basis
CuO
208841
Copper(II) oxide, powder, <10 μm, 98%
CuO
241741
Copper(II) oxide, ACS reagent, ≥99.0%
CuO
203130
Copper(II) oxide, 99.999% trace metals basis
CuO
310433
Copper(II) oxide, needles, mixture of CuO and Cu2O, ACS reagent
CuO
61200
Copper(II) oxide, puriss. p.a., ≥99.0% (RT), powder
CuO
94737
Copper(II) oxide, ≥99.0% (RT), granular
CuO
450804
Copper(II) oxide, powder, 99.995% trace metals basis
CuO
417971
Copper(II) oxide on alumina, 14-30 mesh, extent of labeling: 13 wt. % loading
CuO
203823
Molybdenum, powder, <150 μm, 99.99% trace metals basis
Mo
266892
Molybdenum, powder, <150 μm, 99.9% trace metals basis
Mo
510092
Molybdenum, powder, 1-5 μm, ≥99.9% trace metals basis
Mo
266922
Molybdenum, foil, thickness 0.1 mm, ≥99.9% trace metals basis
Mo
357227
Molybdenum, foil, thickness 0.025 mm, ≥99.9% trace metals basis
Mo
266914
Molybdenum, wire, diam. 1.0 mm, 99.95% trace metals basis
Mo
577987
Molybdenum, nanopowder, <100 nm particle size (TEM), 99.8% trace metals basis
Mo
357200
Molybdenum, foil, thickness 1.0 mm, ≥99.9% trace metals basis
Mo
GF54155055
Molybdenum, foil, 1m coil, thickness 0.01mm, 99.9%
Mo
366986
Molybdenum, powder, 10 μm, ≥99.95% trace metals basis
Mo
GF93556693
Molybdenum, foil, 6mm disks, thickness 0.005mm, 99.9%
Mo
GF04293446
Molybdenum, tube, 500mm, outside diameter 3.15mm, inside diameter 2.39mm, wall thickness 0.38mm, 99.9%
Mo
514802
Molybdenum, foil, thickness 0.05 mm, ≥99.9% trace metals basis
Mo
GF30193781
Molybdenum, foil, not light tested, 50x50mm, thickness 0.0125mm, 99.9%
Mo
GF90875414
Molybdenum, wire reel, 5m, diameter 1.25mm, annealed, 99.95%
Mo
GF76876464
Molybdenum, wire reel, 1m, diameter 0.5mm, annealed, 99.95%
Mo
GF13141572
Molybdenum, foil, 6mm disks, thickness 0.5mm, annealed, 99.9%
Mo
GF68352330
Molybdenum, foil, 6mm disks, thickness 0.75mm, annealed, 99.9%
Mo
GF94586372
Molybdenum, foil, 8mm disks, thickness 0.005mm, 99.9%
Mo
GF30128576
Molybdenum, foil, 8mm disks, thickness 0.006mm, 99.9%
Mo