EMAIL THIS PAGE TO A FRIEND

Die Pharmazie

Defining the role of MRP-mediated efflux and glutathione in detoxification of oxaliplatin.


PMID 23923647

Abstract

Albeit platinum complexes are widely used in cancer chemotherapy, their cellular processing has not been completely elucidated so far. In this study the effects of modulating multidrug resistance-associated protein (MRP)-mediated efflux and glutathione (GSH) depletion on the cytotoxicity of oxaliplatin were assessed in a human ileocecal colorectal adenocarcinoma cell line and its oxaliplatin-resistant variant. Upon oxaliplatin exposure, DNA platination was elevated by co-incubation with Gü83, a MRP1 and MRP2 inhibitor, but cytotoxicity was not increased. Addition of oxaliplatin did not alter the cellular GSH content. Following GSH depletion, platinum accumulation was unchanged but cytotoxicity was increased in oxaliplatin-sensitive cells. In conclusion, modulation of MRP-mediated efflux did not affect oxaliplatin cytotoxicity in the investigated cell lines. Intracellular GSH depletion seems to sensitize the cells but does not overcome resistance.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

373214
Platinum, evaporation slug, diam. × L 0.6 cm × 1.2 cm, 99.99% trace metals basis
Pt
373222
Platinum, evaporation slug, diam. × L 0.6 cm × 0.6 cm, 99.99% trace metals basis
Pt
373230
Platinum, evaporation slug, diam. × L 0.3 cm × 0.6 cm, 99.99% trace metals basis
Pt
349372
Platinum, foil, thickness 1.0 mm, 99.99% trace metals basis
Pt
267260
Platinum, foil, thickness 0.5 mm, 99.99% trace metals basis
Pt
305189
Platinum, foil, thickness 0.5 mm, 99.9% trace metals basis
Pt
349321
Platinum, foil, thickness 0.25 mm, 99.99% trace metals basis
Pt
327425
Platinum, foil, thickness 0.25 mm, 99.9% trace metals basis
Pt
349348
Platinum, foil, thickness 0.127 mm, 99.99% trace metals basis
Pt
327433
Platinum, foil, thickness 0.125-0.135 mm, 99.9% trace metals basis
Pt
267252
Platinum, foil, thickness 0.1 mm, 99.99% trace metals basis
Pt
349380
Platinum, foil, thickness 0.1 mm, 99.9% trace metals basis
Pt
349356
Platinum, foil, thickness 0.05 mm, 99.99% trace metals basis
Pt
303798
Platinum, foil, thickness 0.05 mm, ≥99.9% trace metals basis
Pt
349364
Platinum, foil, thickness 0.025 mm, 99.99% trace metals basis
Pt
267244
Platinum, foil, thickness 0.025 mm, 99.9% trace metals basis
Pt
298107
Platinum, gauze, 52 mesh, 99.9% trace metals basis
Pt
298093
Platinum, gauze, 100 mesh, 99.9% trace metals basis
Pt
685453
Platinum, nanopowder, <50 nm particle size (TEM)
Pt
204048
Platinum, powder (coarse), 99.99% trace metals basis
Pt
204013
Platinum, powder, 99.995% trace metals basis
Pt
327476
Platinum, powder, ≤10 μm, 99.9% trace metals basis
Pt
327484
Platinum, shot, ≤3 mm, ≥99.9% trace metals basis
Pt
267155
Platinum, sponge, ≥99.9% trace metals basis
Pt
444685
Platinum, wire, diam. 2.0 mm, 99.9% trace metals basis
Pt
349399
Platinum, wire, diam. 1.5 mm, ≥99.9% trace metals basis
Pt
267236
Platinum, wire, diam. 1.0 mm, 99.99% trace metals basis
Pt
327492
Platinum, wire, diam. 1.0 mm, 99.9% trace metals basis
Pt
267201
Platinum, wire, diam. 0.5 mm, 99.99% trace metals basis
Pt
267228
Platinum, wire, diam. 0.5 mm, 99.9% trace metals basis
Pt