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Diagnostic microbiology and infectious disease

HindII and SduI digests of heat-shock protein 70 PCR for Leishmania typing.


PMID 24050933

Abstract

Restriction fragment length polymorphisms of the heat-shock protein 70 gene have been used for discriminating Leishmania species. Here, we validated HindII as a much cheaper alternative to EcoRII and SduI for discriminating Leishmania (Viannia) braziliensis from Leishmania (Viannia) naiffi and an atypical Leishmania (V.) braziliensis group, which was previously not possible.