EMAIL THIS PAGE TO A FRIEND

Methods in molecular biology (Clifton, N.J.)

Photoswitchable fluorescent proteins for superresolution fluorescence microscopy circumventing the diffraction limit of light.


PMID 24108655

Abstract

In the last two decades, fluorescent proteins became an indispensable tool to noninvasively label a protein in living cells. The discovery of photoswitchable fluorescent proteins expanded the applications of the fluorescent proteins to techniques such as molecular tracking and highlighting on a microscope. Recently, a new microscopic modality to achieve a superresolution circumventing the diffraction limit of light (photoactivated localization microscopy, PALM) has been developed based on the photoswitchable fluorescent proteins. Here we introduce a basic protocol of PALM through the visualization of actin bundles with superresolution.