EMAIL THIS PAGE TO A FRIEND

In vitro cellular & developmental biology : journal of the Tissue Culture Association

Primary cultures of rat pancreatic acinar cells in serum-free medium.


PMID 2418006

Abstract

Rat pancreatic acinar cells were isolated and cultured in Ham's F12 medium with 15% bovine calf serum. Caerulein, insulin, somatostatin, and dexamethasone (DEX) had no effect on intracellular or secreted amylase in these cultured cells. A serum-free medium, using Waymouth's MB 752/1 supplemented with albumin, epidermal growth factor (EGF), DEX, and HEPES, was then developed to avoid serum factors that might mask hormonal effects. In this SF medium, pancreatic acinar cells maintained the morphological and ultrastructural characteristics of freshly isolated cells and secreted amylase in response to the secretagogue, carbamyl choline. Insulin, at a concentration of 1 microgram/ml, significantly increased intracellular and secreted amylase activity after 3 d. This model cell system can be used to study the regulation of the synthesis of amylase and other pancreatic enzymes in vitro.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

54461
HEPES, ≥99.0% (T)
C8H18N2O4S