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Biochemical and biophysical research communications

Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system.


PMID 24491566

Abstract

The mammalian zygote-mediated CRISPR/Cas system can efficiently generate targeted genome-modified animals. However, this system is limited by the risk of off-target mutations. Here we show that offset-nicking by Cas9 nickase and paired gRNAs allows us to generate region deleted mice and targeted knock-in mice without off-target mutations.