EMAIL THIS PAGE TO A FRIEND

Nature communications

Dynamic phosphorylation of HP1α regulates mitotic progression in human cells.


PMID 24619172

Abstract

Heterochromatin protein 1α (HP1α), a key player in the establishment and maintenance of higher-order chromatin regulates key cellular processes, including metaphase chromatid cohesion and centromere organization. However, how HP1α controls these processes is not well understood. Here we demonstrate that post-translational modifications of HP1α dictate its mitotic functions. HP1α is constitutively phosphorylated within its amino terminus, whereas phosphorylation within the hinge domain occurs preferentially at G2/M phase of the cell cycle. The hinge-phosphorylated form of HP1α specifically localizes to kinetochores during early mitosis and this phosphorylation mediated by NDR1 kinase is required for mitotic progression and for Sgo1 binding to mitotic centromeres. Cells lacking NDR kinase show loss of mitosis-specific phosphorylation of HP1α leading to prometaphase arrest. Our results reveal that NDR kinase catalyses the hinge-specific phosphorylation of human HP1α during G2/M in vivo and this orchestrates accurate chromosome alignment and mitotic progression.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

MAB3448
Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9, Use Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b.
MAB3584
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5, Use Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5 (mouse monoclonal antibody) validated in ELISA, ICC, IHC to detect Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5.
MAB3446
Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9, Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.
MAB3450
Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6, Anti-Heterochromatin Protein-1 gamma Antibody, clone 2MOD-1G6 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 gamma also known as HP-1gamma & has been validated in ELISA, ICC, IF, IHC & WB.
MABS56
Anti-NDR1/2 protein kinase Antibody, clone BH6-31G14, Anti-NDR1/2 protein kinase Antibody, clone BH6-31G14 is an antibody against NDR1/2 protein kinase for use in WB, ELISA.
06-570
Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker, Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker is a Rabbit Polyclonal Antibody for detection of Histone H3 phosphorylated at serine 10.This highly published Ab, also known as Anti-H3S10p, has been validated in ICC, IP & WB.
07-523
Anti-trimethyl-Histone H3 (Lys9) Antibody, Anti-trimethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of trimethyl-Histone H3 (Lys9) also known as H3K9me3, Histone H3 (tri methyl K9) & has been validated in DB, PIA & WB.