EMAIL THIS PAGE TO A FRIEND

FEBS letters

Pyrophosphate analogues in pyrophosphorolysis reaction catalyzed by DNA polymerases.


PMID 2469598

Abstract

It is demonstrated here that rat liver DNA polymerase beta catalyzes the pyrophosphorolysis reaction with pyrophosphate (PPi) and its analogues. The substrate specificity of the PPi-binding site of several DNA polymerases was investigated. It was discovered that the ability of DNA polymerases to utilize PPi analogues instead of PPi in the pyrophosphorolysis reaction was markedly restricted. Only imidodiphosphate and methylenediphosphonate were demonstrated as participating in this process. Oxodiphosphonate and phosphonoformate inhibited DNA synthesis, but probably not via the interaction with the PPi-binding site of DNA polymerases.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

I0631
Imidodiphosphate sodium salt, ≥97%
HNNa4O6P2