EMAIL THIS PAGE TO A FRIEND

Experimental neurology

Radial glial progenitors repair the zebrafish spinal cord following transection.


PMID 24721238

Abstract

In mammals, spinal cord injury results in permanent sensory-motor loss due in part to a failure in reinitiating local neurogenesis. However, zebrafish show robust neuronal regeneration and functional recovery even after complete spinal cord transection. Postembryonic neurogenesis is dependent upon resident multipotent progenitors, which have been identified in multiple vertebrates. One candidate cell population for injury repair expresses Dbx1, which has been shown to label multipotent progenitors in mammals. In this study, we use specific markers to show that cells expressing a dbx1a:GFP reporter in the zebrafish spinal cord are radial glial progenitors that continue to generate neurons after embryogenesis. We also use a novel larval spinal cord transection assay to show that dbx1a:GFP(+) cells exhibit a proliferative and neurogenic response to injury, and contribute newly-born neurons to the regenerative blastema. Together, our data indicate that dbx1a:GFP(+) radial glia may be stem cells for the regeneration of interneurons following spinal cord injury in zebrafish.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

AV03018
Anti-PCNA antibody produced in rabbit, IgG fraction of antiserum
HPA030521
Anti-PCNA antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, ab1
HPA030522
Anti-PCNA antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, ab2
HPA030523
Anti-PCNA antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, ab3
SAB2701819
Anti-PCNA antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB4502103
Anti-PCNA antibody produced in rabbit, affinity isolated antibody
AV38648
Anti-SOX3 (AB1) antibody produced in rabbit, IgG fraction of antiserum
AV38649
Anti-SOX3 (AB2) antibody produced in rabbit, IgG fraction of antiserum
S7443 Anti-Sox3 antibody produced in rabbit, affinity isolated antibody, ammonium sulfate suspension
AV33321
Anti-SOX3 antibody produced in rabbit, affinity isolated antibody
SAB2104042
Anti-SOX3 antibody produced in rabbit, affinity isolated antibody
A5040
Ethyl 3-aminobenzoate methanesulfonate salt, analytical standard
C9H11NO2 · CH4SO3
WH0005111M2
Monoclonal Anti-PCNA antibody produced in mouse, clone 1G7, purified immunoglobulin, buffered aqueous solution
SAB1404178
Monoclonal Anti-PCNA antibody produced in mouse, clone S1, purified immunoglobulin, buffered aqueous solution
SAB4100556
Monoclonal Anti-PCNA antibody produced in mouse, clone PCNA -A509, culture supernatant
PLA0079
Rabbit anti-PCNA Antibody, Affinity Purified, Powered by Bethyl Laboratories, Inc.
PLA0080
Rabbit anti-PCNA Antibody, Affinity Purified, Powered by Bethyl Laboratories, Inc.