EMAIL THIS PAGE TO A FRIEND

Human molecular genetics

Formin 1 and filamin B physically interact to coordinate chondrocyte proliferation and differentiation in the growth plate.


PMID 24760772

Abstract

Filamin B (FlnB) is an actin-binding protein thought to transduce signals from various membrane receptors and intracellular proteins onto the actin cytoskeleton. Formin1 (Fmn1) is an actin-nucleating protein, implicated in actin assembly and intracellular signaling. Human mutations in FLNB cause several skeletal disorders associated with dwarfism and early bone fusion. Mouse mutations in Fmn1 cause aberrant fusion of carpal digits. We report here that FlnB and Fmn1 physically interact, are co-expressed in chondrocytes in the growth plate and share overlapping expression in the cell cytoplasm and nucleus. Loss of FlnB leads to a dramatic decrease in Fmn1 expression at the hypertrophic-to-ossification border. Loss of Fmn1-FlnB in mice leads to a more severe reduction in body size, weight and growth plate length, than observed in mice following knockout of either gene alone. Shortening of the long bone is associated with a decrease in chondrocyte proliferation and an overall delay in ossification in the double-knockout mice. In contrast to FlnB null, Fmn1 loss results in a decrease in the width of the prehypertrophic zone. Loss of both proteins, however, causes an overall decrease in the width of the proliferation zone and an increase in the differentiated hypertrophic zone. The current findings suggest that Fmn1 and FlnB have shared and independent functions. FlnB loss promotes prehypertrophic differentiation whereas Fmn1 leads to a delay. Both proteins, however, regulate chondrocyte proliferation, and FlnB may regulate Fmn1 function at the hypertrophic-to-ossification border, thereby explaining the overall delay in ossification.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

06-570
Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker, Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker is a Rabbit Polyclonal Antibody for detection of Histone H3 phosphorylated at serine 10.This highly published Ab, also known as Anti-H3S10p, has been validated in ICC, IP & WB.
HPA001758
Anti-SOX9 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV37986
Anti-SOX9 antibody produced in rabbit, affinity isolated antibody
AV100797
Anti-SOX9 antibody produced in rabbit, IgG fraction of antiserum
SAB4502834
Anti-SOX9 antibody produced in rabbit, affinity isolated antibody
HHS32
Hematoxylin Solution, Harris Modified
11074547001 Insulin-Transferrin-Sodium Selenite Supplement
SAB3300002
Monoclonal Anti-c-Myc antibody produced in mouse, clone 1A6, purified immunoglobulin, buffered aqueous glycerol solution
WH0001280M1
Monoclonal Anti-COL2A1 antibody produced in mouse, clone 3H1-F9, purified immunoglobulin, buffered aqueous solution
SAB1403684
Monoclonal Anti-COL2A1 antibody produced in mouse, clone 1E1, purified immunoglobulin, buffered aqueous solution
WH0004609M2
Monoclonal Anti-MYC antibody produced in mouse, clone 1G7, purified immunoglobulin, buffered aqueous solution
S9133
Sodium selenite, γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture
Na2O3Se
S5261
Sodium selenite, BioReagent, suitable for cell culture, ≥98%
Na2O3Se
71950
Sodium selenite, anhydrous, ≥95.0% (RT)
Na2O3Se
214485
Sodium selenite, 99%
Na2O3Se