EMAIL THIS PAGE TO A FRIEND

The international journal of biochemistry & cell biology

Molecular and cellular basis of the regulation of lymphatic contractility and lymphatic absorption.


PMID 24836907

Abstract

Lymphatic absorption is a highly regulated process driven by both an extrinsic mechanism (external force) and an intrinsic mechanism (lymphatic vessel contractility). The lymphatic muscle is a specialized smooth muscle with unique mechanical properties. To understand the molecular mechanism and relative contribution of smooth muscle contraction in lymphatic absorption, we analyzed mice with a smooth muscle-specific deletion of Mylk, a critical gene for smooth muscle contraction. Interestingly, the knockout mice were significantly resistant to anesthesia reagents. Upon injection in the feet with FITC-dextran, the mutant mice displayed a 2-fold delay of the absorption peak in the peripheral circulation. Examining the ear lymphatic vessels of the mutant mice revealed a reduction in the amount of fluid in the lumens of the lymphangions, suggesting an impairment of lymph formation. The Mylk-deficient lymphatic muscle exhibited a significant reduction of peristalsis and of myosin light chain phosphorylation in response to depolarization. We thus concluded that MLCK and myosin light chain phosphorylation are required for lymphatic vessel contraction. Lymphatic contractility is not an exclusive requirement for lymphatic absorption, and external force appears to be necessary for absorption.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1300116
Anti-MLCK (N-term) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
SAB1305415
ANTI-MLCK(N-TERMINAL) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
SAB2501937
Anti-MYLK antibody produced in goat, affinity isolated antibody, buffered aqueous solution
HPA031677
Anti-MYLK antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1410811
Anti-MYLK antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB1105884
Anti-MYLK2 (181-195) antibody produced in rabbit, IgG fraction of antiserum
SAB1409913
Anti-MYLK2 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA059704
Anti-MYLK2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA059890
Anti-MYLK2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1411532
Anti-MYLK2 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB1305373
ANTI-MYLK3(N-TERMINAL) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
WH0004638M1
Monoclonal Anti-MYLK antibody produced in mouse, clone 1D1, purified immunoglobulin, buffered aqueous solution
WH0085366M1
Monoclonal Anti-MYLK2 antibody produced in mouse, clone 2G1, purified immunoglobulin, buffered aqueous solution
M7905
Monoclonal Anti-Myosin Light Chain Kinase antibody produced in mouse, clone K36, ascites fluid
SAB1305112
ANTI-MYLK3 (CENTER) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution