American journal of reproductive immunology (New York, N.Y. : 1989)

Investigation of galectin-3 function in the reproductive tract by identification of binding ligands in human seminal plasma.

PMID 24863808


Galectin-3 is a β-galactoside binding protein with immunomodulatory properties and exerts its extracellular functions via interactions with glycoconjugate ligands. Therefore, to elucidate the function of galectin-3, binding ligands in human seminal plasma were investigated. Galectin-3 binding proteins were isolated from seminal plasma by affinity chromatography, and candidate ligands were identified by MS/MS. Biochemical methods were used to characterize the ability of galectin-3 to bind its ligands. Identified galectin-3 ligands included CD13, MUC6, PAP, PSA, and ZAG. 1D and 2D electrophoretic analysis of seminal plasma demonstrated that CD13, PAP, PSA, and ZAG immunoreactivity co-migrated with galectin-3-reactive protein bands and spots at expected molecular weights and pIs. Inhibition assays indicated that CD13, PSA, PAP, and ZAG interact with galectin-3 in a protein-carbohydrate manner. The galectin-3 binding ligands identified in this study indicate multiple roles for galectin-3 in the reproductive and immunological functions of seminal plasma.

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G5170 Galectin-3 human, recombinant, expressed in E. coli, lyophilized powder