EMAIL THIS PAGE TO A FRIEND

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology

Evaluation of a porcine model of early aortic valve sclerosis.


PMID 24998316

Abstract

Calcific aortic valve disease (CAVD) is associated with significant cardiovascular morbidity. While late-stage CAVD is well-described, early pathobiological processes are poorly understood due to the lack of animal models that faithfully replicate early human disease. Here we evaluated a hypercholesterolemic porcine model of early diet-induced aortic valve sclerosis. Yorkshire swine were fed either a standard or high-fat/high-cholesterol diet for 2 or 5 months. Right coronary aortic valve leaflets were excised and analyzed (immuno)histochemically. Early human-like proteoglycan-rich onlays formed between the endothelial layer and elastic lamina in the fibrosa layer of valve leaflets, with accelerated formation associated with hypercholesterolemia (P<.05). Lipid deposition was more abundant in hypercholesterolemic swine (P<.001), but was present in a minority (28%) of onlays. No myofibroblasts, MAC387-positive macrophages, or fascin-positive dendritic cells were detected in 2-month onlays, with only scarce myofibroblasts present at 5 months. Cells that expressed osteochondral markers Sox9 and Msx2 were preferentially found in dense proteoglycan-rich onlays (P<.05) and with hypercholesterolemia (P<.05). Features of more advanced human CAVD, including calcification, were not observed in this necessarily short study. Early aortic valve sclerosis in hypercholesterolemic swine is characterized by the formation of proteoglycan-rich onlays in the fibrosa, which can occur prior to significant lipid accumulation, inflammatory cell infiltration, or myofibroblast activation. These characteristics mimic those of early human aortic valve disease, and thus the porcine model has utility for the study of early valve sclerosis.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB2501669
Anti-CASP3 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
HPA002643
Anti-CASP3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV00021
Anti-CASP3 antibody produced in rabbit, IgG fraction of antiserum
SAB4503291
Anti-CASP3 antibody produced in rabbit, affinity isolated antibody
C9598 Anti-Caspase 3 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
HPA005723
Anti-FSCN1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2700218
Anti-FSCN1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2106726
Anti-FSCN1 antibody produced in rabbit, affinity isolated antibody
HPA000451
Anti-MKI67 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA001164
Anti-MKI67 antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV31396
Anti-MSX1 (AB1) antibody produced in rabbit, IgG fraction of antiserum
AV37094
Anti-MSX1 (AB2) antibody produced in rabbit, IgG fraction of antiserum
SAB2500650 Anti-MSX1 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB1412289
ANTI-MSX1 antibody produced in mouse, clone 3A8, purified immunoglobulin, buffered aqueous solution
M0944 Anti-Msx1 antibody produced in rabbit, affinity isolated antibody, ammonium sulfate suspension
M6198
Anti-MSX1 antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
WH0000836M2
Monoclonal Anti-CASP3 antibody produced in mouse, clone 4D3, purified immunoglobulin, buffered aqueous solution
C5737 Monoclonal Anti-Caspase 3 antibody produced in mouse, clone 84803.111, purified immunoglobulin, lyophilized powder
WH0004288M1
Monoclonal Anti-MKI67 antibody produced in mouse, clone 7B8, purified immunoglobulin, buffered aqueous solution
AMAB90870
Monoclonal Anti-MKI67 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL1234, purified immunoglobulin, buffered aqueous glycerol solution
SAB5300423 Monoclonal Anti-MKI67 antibody produced in mouse, clone 4A1, ascites fluid
SAB5300424 Monoclonal Anti-MKI67 antibody produced in mouse, clone 9C12B2, ascites fluid
SAB5300425 Monoclonal Anti-MKI67 antibody produced in mouse, clone 8D5, ascites fluid
WH0004487M3
Monoclonal Anti-MSX1 antibody produced in mouse, clone 2B12, purified immunoglobulin, buffered aqueous solution
SAB1404087
Monoclonal Anti-MSX1 antibody produced in mouse, clone 1D2, purified immunoglobulin, buffered aqueous solution
SAB5300418
Monoclonal Anti-MSX1 antibody produced in mouse, clone 5D11, ascites fluid