EMAIL THIS PAGE TO A FRIEND

Molecular and cellular neurosciences

Intracellular alpha-synuclein affects early maturation of primary oligodendrocyte progenitor cells.


PMID 25019582

Abstract

Myelin loss is a widespread neuropathological hallmark of the atypical parkinsonian disorder multiple system atrophy (MSA). On a cellular level, MSA is characterized by alpha-synuclein (aSyn)-positive glial cytoplasmic inclusions (GCIs) within mature oligodendrocytes leading to demyelination as well as axonal and neuronal loss. Oligodendrocyte progenitor cells (OPCs) represent a proliferative cell population distributed throughout the adult mammalian central nervous system. During remyelination, OPCs are recruited to sites of demyelination, differentiate, and finally replace dysfunctional mature oligodendrocytes. However, comprehensive studies investigating OPCs and remyelination processes in MSA are lacking. In the present study, we therefore investigate the effect of human aSyn (h-aSyn) on early primary rat OPC maturation. Upon lentiviral transduction, h-aSyn expressing OPCs exhibit fewer and shorter primary processes at the initiation of differentiation. Until day 4 of a 6day differentiation paradigm, h-aSyn expressing OPCs further show a severely delayed maturation evidenced by reduced myelin gene expression and increased levels of the progenitor marker platelet derived growth factor receptor-alpha (PDGFRα). Matching these results, OPCs that take up extracellular recombinant h-aSyn exhibit a similar delayed differentiation. In both experimental setups however, myelin gene expression is restored at day 6 of differentiation paralleled by decreased intracellular h-aSyn levels indicating a reverse correlation of h-aSyn and the differentiation potential of OPCs. Taken together, these findings suggest a tight link between the intracellular level of h-aSyn and maturation capacity of primary OPCs.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

AV32753
Anti-OLIG2 (AB1) antibody produced in rabbit, affinity isolated antibody
AV31464
Anti-OLIG2 (AB2) antibody produced in rabbit, affinity isolated antibody
AV32752
Anti-OLIG2 (AB3) antibody produced in rabbit, affinity isolated antibody
SAB1412293
ANTI-OLIG2 antibody produced in mouse, clone 2A8, purified immunoglobulin, buffered aqueous solution
SAB1412294
ANTI-OLIG2 antibody produced in mouse, clone 2B11, purified immunoglobulin, buffered aqueous solution
HPA003254
Anti-OLIG2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1406585
Anti-VIM antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
WH0010215M2
Monoclonal Anti-OLIG2 antibody produced in mouse, clone 3D7, purified immunoglobulin, buffered aqueous solution
SAB1404798
Monoclonal Anti-OLIG2 antibody produced in mouse, clone 3C9, purified immunoglobulin, buffered aqueous solution
SAB5300407
Monoclonal Anti-OLIG2 antibody produced in mouse, clone 1G11, ascites fluid
WH0007431M1
Monoclonal Anti-VIM antibody produced in mouse, clone 3E9, purified immunoglobulin, buffered aqueous solution
SAB4700524 Monoclonal Anti-VIM antibody produced in mouse, clone VI-RE/1, purified immunoglobulin, buffered aqueous solution
SAB4100612
Monoclonal Anti-VIM antibody produced in mouse, clone VIM-A604, culture supernatant
AMAB90516
Monoclonal Anti-VIM antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL0157, purified immunoglobulin, buffered aqueous glycerol solution