EMAIL THIS PAGE TO A FRIEND

Journal of chromatography. A

Urinary metabolic fingerprinting of mice with diet-induced metabolic derangements by parallel dual secondary column-dual detection two-dimensional comprehensive gas chromatography.


PMID 25160948

Abstract

This study investigates the potential of a parallel dual secondary column-dual detection two-dimensional comprehensive GC platform (GC×2GC-MS/FID) for metabolic profiling and fingerprinting of mouse urine. Samples were obtained from a murine model that mimics a typical unhealthy Western diet featuring both high fat and sugar (HFHS) intake, which induces obesity, dyslipidemia, and insulin resistance. Urines collected at different steps of the study were used to obtain pivotal and comparative data on the presence and relative distributions of early markers of metabolic disease. The data elaboration and interpretation work-flow includes an advanced untargeted fingerprinting approach, with peak-region features to locate relevant features to be quantified by external standard calibration. The reliability of untargeted fingerprinting is confirmed by quantitative results on selected relevant features that showed percentages of variations consistent with those observed by comparing raw data quantitative descriptors (2D peak-region volumes and percent of response). Analytes that were up-regulated with % of variation ranging from 30 to 1000, included pyruvic acid, glycerol, fructose, galactose, glucose, lactic acid, mannitol and valine. Down-regulation was evidenced for malonic acid, succinic acid, alanine, glycine, and creatinine. Advanced fingerprinting also is demonstrated for effectively evaluating individual variations during experiments, thus representing a promising tool for personalized intervention studies. In this context, it is interesting to observe that informative features that were not discriminant for the entire population may be relevant for individuals.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

K1750
α-Ketoglutaric acid, ≥98.5% (NaOH, titration)
C5H6O5
75890
α-Ketoglutaric acid, 99.0-101.0% (T)
C5H6O5
K1128
α-Ketoglutaric acid, BioReagent, suitable for cell culture, suitable for insect cell culture
C5H6O5
320102
Acetic anhydride, ReagentPlus®, ≥99%
C4H6O3
242845
Acetic anhydride, ACS reagent, ≥98.0%
C4H6O3
539996
Acetic anhydride, 99.5%
C4H6O3
691275
Acetic anhydride, Lonza quality, ≥99.5% (GC)
C4H6O3
L095004
Acetic anhydride
C4H6O3
A5502
Adonitol, ≥99%
C5H12O5
02240
Adonitol, BioXtra, ≥99.0% (HPLC)
C5H12O5
G0750
D-(+)-Galactose, ≥99%
C6H12O6
G0625
D-(+)-Galactose, ≥98%
C6H12O6
G5388
D-(+)-Galactose, powder, anhydrous, BioReagent, suitable for cell culture, suitable for insect cell culture
C6H12O6
48260
D-(+)-Galactose, for microbiology, ≥99.0%
C6H12O6
G6404
D-(+)-Galactose, BioXtra, pH 5.0-7.0 (20 °C, 1 M in H2O), ≥99%
C6H12O6
15522
D-(+)-Galactose, meets analytical specification of Ph. Eur., BP
C6H12O6
M4125
D-Mannitol, ≥98%
C6H14O6
M9647
D-Mannitol, ACS reagent
C6H14O6
M8429
D-Mannitol, meets EP, FCC, USP testing specifications
C6H14O6
M9546
D-Mannitol, BioXtra, ≥98% (HPLC)
C6H14O6
M1902
D-Mannitol, suitable for plant cell culture
C6H14O6
63559
D-Mannitol, BioUltra, ≥99.0% (sum of enantiomers, HPLC)
C6H14O6
63560
D-Mannitol, ACS reagent, for microbiology, ≥99.0%
C6H14O6
17311
D-Mannitol, tested according to Ph Eur
C6H14O6
78513
D-Mannitol, ≥99.9999% (metals basis), for boron determination
C6H14O6
F0127
D-(−)-Fructose, ≥99%
C6H12O6
F3510
D-(−)-Fructose, BioReagent, suitable for cell culture, suitable for insect cell culture
C6H12O6
F2543
D-(−)-Fructose, BioXtra
C6H12O6
47739
D-(−)-Fructose, BioUltra, ≥99.0% (HPLC)
C6H12O6
F9048
D-(−)-Fructose, meets USP testing specifications
C6H12O6