EMAIL THIS PAGE TO A FRIEND

Phytomedicine : international journal of phytotherapy and phytopharmacology

Chemical composition and free radical-scavenging, anticancer and anti-inflammatory activities of the essential oil from Ocimum kilimandscharicum.


PMID 25172793

Abstract

The essential oil from the leaves of Ocimum kilimandscharicum (EOOK), collected in Dourados-MS, was investigated for anticancer, anti-inflammatory and antioxidant activity and chemical composition. The essential oil was extracted by hydrodistillation, and the chemical composition was performed by gas chromatography-mass spectrometry. The essential oil was evaluated for free radical-scavenging activity using the DPPH assay and was tested in an anticancer assay against ten human cancer cell lines. The response parameter (GI50) was calculated for the cell lines tested. The anti-inflammatory activity was evaluated using carrageenan-induced pleurisy in mice. The chemical composition showed 45 components with a predominance of monoterpenes, such as camphor (51.81%), 1,8 cineole (20.13%) and limonene (11.23%). The EOOK exhibited potent free radical-scavenging activity by the DPPH assay with a GI50 of 8.31 μg/ml. The major constituents, pure camphor (IC50=12.56 μg/ml) and mixture of the limonene: 1, 8 cineole (IC50=23.25 μg/ml) displayed a potent activity. The oral administration of EOOK (at 30 and 100 mg kg(-1)), as well as the pure camphor or a mixture of 1,8 cineole with limonene, significantly inhibited the carrageenan (Cg) induced pleurisy, reducing the migration of total leukocytes in mice by 82 ± 4% (30 mg kg(-1) of EOOK), 95 ± 4% (100 mg kg(-1) of EOOK), 83 ± 9% (camphor) and 80 ± 5% (mixture of 1,8 cineole:limonene 1:1). In vitro cytotoxicity screening against a human ovarian cancer cell line displayed high selectivity and potent anticancer activity with GI50=31.90 mg ml(-1). This work describes the anti-inflammatory, anticancer and antioxidant effects of EOOK for the first time. The essential oil exhibited marked anti-inflammatory, antioxidant and anticancer effects, an effect that can be attributed the presence of majorital compounds, and the response profiles from chemical composition differed from other oils collected in different locales.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

21310
(±)-Camphor, purum, synthetic, ≥95.0% (GC)
C10H16O
20506
(±)-Camphor, meets analytical specification of Ph. Eur., BP, ≥95% (GC), racemic
C10H16O
W526606
(±)-Camphor, ≥95.5%
C10H16O
21300
(+)-Camphor, purum, natural, ≥97.0% (sum of enantiomers, GC)
C10H16O
21293
(−)-Camphor, analytical standard
C10H16O
21295
(−)-Camphor, purum, ≥95.0% (sum of enantiomers, GC)
C10H16O
00020590
1,8-Cineole, primary reference standard
C10H18O
B1378
2,6-Di-tert-butyl-4-methylphenol, ≥99.0% (GC), powder
C15H24O
34750
2,6-Di-tert-butyl-4-methylphenol, purum, ≥99.0% (GC)
C15H24O
03762
2,6-Di-tert-butyl-4-methylphenol, tested according to Ph Eur
C15H24O
1082708
Butylated hydroxytoluene, United States Pharmacopeia (USP) Reference Standard
C15H24O
W218405
Butylated hydroxytoluene, ≥99%, FCC, FG
C15H24O
PHR1117
Butylated Hydroxytoluene, Pharmaceutical Secondary Standard; Certified Reference Material
C15H24O
1087508
Camphor, United States Pharmacopeia (USP) Reference Standard
C10H16O
148075
Camphor, 96%
C10H16O
Y0000176
Cineole, European Pharmacopoeia (EP) Reference Standard
C10H18O
W524905
Dipentene, mixture of isomers
C10H16
334111
Dipentene, technical, for use as solvent (for the paint industry), mixture of various terpenes
C10H16
29210
Eucalyptol, analytical standard
C10H18O
95656
Eucalyptol, certified reference material, TraceCERT®
C10H18O
C80601
Eucalyptol, 99%
C10H18O
W246506
Eucalyptol, natural, ≥99%, FCC, FG
C10H18O
T0501
Thymol, ≥98.5%
C10H14O
T1350000
Thymol, European Pharmacopoeia (EP) Reference Standard
C10H14O
PHR1134
Thymol, Pharmaceutical Secondary Standard; Certified Reference Material
C10H14O
16254
Thymol, meets analytical specification of Ph. Eur., BP, NF, 99-101%
C10H14O
50409
Thymol, Standard for quantitative NMR, TraceCERT®
C10H14O
72477
Thymol, analytical standard
C10H14O
00670590
Thymol, primary reference standard
C10H14O
89329
Thymol, tested according to Ph Eur
C10H14O