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Methods in molecular biology (Clifton, N.J.)

Headspace SPME-GC-MS metabolomics analysis of urinary volatile organic compounds (VOCs).


PMID 25270935

Abstract

In this chapter, we describe the procedure for metabolic profiling of hundreds of urinary volatile organic compounds (VOCs) using headspace solid-phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS). SPME is a solvent-free technology that is reproducible, fast, cost effective, and versatile in extracting small molecular weight organic compounds (metabolites) from biofluids using a fiber that is coated with an extracting phase. After extraction, the SPME fiber can be directly injected to the GC-MS, where the extracted metabolites will desorb thermally from the fiber, elute along a GC column, and finally enter into the MS for detection. The analysis of urine samples is presented using this approach.