Biotechnology letters

Comparison of chloromethyl-dialkylcarbocyanine and green fluorescent protein for labeling human umbilical mesenchymal stem cells.

PMID 25280731


To investigate the effectiveness of chloromethyl-dialkylcarbocyanine (CM-Dil) and green florescent protein (GFP) for tracking transplanted stem human umbilical cord-derived mesenchymal stem cells (hUC-MSCs), hUC-MSCs were labeled with CM-Dil or GFP and transplanted into guinea pigs with nasal mucosa radiation injury. In vitro and in vivo labeling efficiency was investigated by flow cytometry and fluorescence microscopy. Proliferation and multi-lineage differentiation potential of labeled hUC-MSCs were assessed via cell quantification and specific staining. In vitro CM-Dil and GFP labeling efficiency was 95 ± 12.2 and 90 ± 8 % at first passage, respectively. Labeled hUC-MSCs were detected by fluorescence microscopy 10 days (CM-Dil) or 20 days (GFP) after transplantation. Neither type of cell labeling affected the multi-differentiation potential but GFP labeling inhibited hUC-MSC proliferation due to virus toxicity. hUC-MSCs can be labeled with either CM-Dil or GFP with high efficiency without impacting multi-differentiation potential.