EMAIL THIS PAGE TO A FRIEND

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Rosmarinic acid attenuates hepatic ischemia and reperfusion injury in rats.


PMID 25455894

Abstract

Rosmarinic acid (RosmA) demonstrates antioxidant and anti-inflammatory properties. We investigated the effect of RosmA on liver ischemia/reperfusion injury. Rats were submitted to 60 min of ischemia plus saline or RosmA treatment (150 mg/kg BW intraperitoneally) followed by 6 h of reperfusion. Hepatocellular injury was evaluated according to aminotransferase activity and histological damage. Hepatic neutrophil accumulation was also evaluated. Oxidative/nitrosative stress was estimated by measuring the reduced glutathione, lipid hydroperoxide and nitrotyrosine levels. Endothelial and inducible nitric oxide synthase (eNOS/iNOS) and nitric oxide (NO) were assessed with immunoblotting and chemiluminescence assays. Hepatic tumor necrosis factor-alpha (TNF-α) and interleukin-1beta mRNA were assessed using real-time PCR, and nuclear factor-kappaB (NF-κB) activation was estimated by immunostaining. RosmA treatment reduced hepatocellular damage, neutrophil infiltration and all oxidative/nitrosative stress parameters. RosmA decreased the liver content of eNOS/iNOS and NO, attenuated NF-κB activation, and down-regulated TNF-α and interleukin-1beta gene expression. These data indicate that RosmA exerts anti-inflammatory and antioxidant effects in the ischemic liver, thereby protecting hepatocytes against ischemia/reperfusion injury. The mechanisms underlying these effects may be related to the inhibitory potential of RosmA on the NF-κB signaling pathway and the reduction of iNOS and eNOS expressions and NO levels, in addition to its natural antioxidant capability.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

N1000
1-Naphthol, ReagentPlus®, ≥99%
C10H8O
31097
1-Naphthol, PESTANAL®, analytical standard
C10H8O
N2780
1-Naphthol, BioXtra, ≥99%
C10H8O
33420
1-Naphthol, puriss. p.a., Reag. Ph. Eur., ≥99% (GC)
C10H8O
1102974
Cetrimonium bromide, United States Pharmacopeia (USP) Reference Standard
C19H42BrN
ARK2188
Hexadecyltrimethylammonium bromide, PharmaGrade, USP/NF, Manufactured under appropriate controls for pharma or biopharmaceutical production.
C19H42BrN
H9151
Hexadecyltrimethylammonium bromide, BioXtra, ≥99%
C19H42BrN
52365
Hexadecyltrimethylammonium bromide, BioUltra, for molecular biology, ≥99.0% (AT)
C19H42BrN
H6269
Hexadecyltrimethylammonium bromide, for molecular biology, ≥99%
C19H42BrN
52367
Hexadecyltrimethylammonium bromide, for ion pair chromatography
C19H42BrN
H5882
Hexadecyltrimethylammonium bromide, ≥98%
C19H42BrN
36932
Hexadecyltrimethylammonium bromide, analytical standard
C19H42BrN
52370
Hexadecyltrimethylammonium bromide, ≥96.0% (AT)
C19H42BrN
07883
Hexadecyltrimethylammonium bromide, puriss. p.a., matrix substance for MALDI-MS
C19H42BrN
72586
Nitrite ion standard solution, 0.1 M NO2-, for ion-selective electrodes
NNaO2
72587
Nitrite ion standard solution, 0.01 M NO2-, for ion-selective electrodes
NNaO2
563218
Sodium nitrite, 99.999% trace metals basis
NNaO2
67398
Sodium nitrite, analytical standard
NNaO2