EMAIL THIS PAGE TO A FRIEND

Environmental research

Genotoxic and cell-transforming effects of titanium dioxide nanoparticles.


PMID 25460650

Abstract

The in vitro genotoxic and the soft-agar anchorage independent cell transformation ability of titanium dioxide nanoparticles (nano-TiO2) and its microparticulated form has been evaluated in human embryonic kidney (HEK293) and in mouse embryonic fibroblast (NIH/3T3) cells. Nano-TiO2 of two different sizes (21 and 50 nm) were used in this study. The comet assay, with and without the use of FPG enzyme, the micronucleus assay and the soft-agar colony assay were used. For both the comet assay and the frequency of micronuclei a statistically significant induction of DNA damage, was observed at the highest dose tested (1000 µg/mL). No oxidative DNA damage induction was observed when the comet assay was complemented with the use of FPG enzyme. Furthermore, long-term exposure to nano-TiO2 has also proved to induce cell-transformation promoting cell-anchorage independent growth in soft-agar. Results were similar for the two nano-TiO2 sizes. Negative results were obtained when the microparticulated form of TiO2 was tested, indicating the existence of important differences between the microparticulated and nanoparticulated forms. As a conclusion it should be indicated that the observed genotoxic/tranforming effects were only detected at the higher dose tested (1000 µg/mL) what play down the real risk of environmental exposures to this nanomaterial.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

348791
Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis
Ti
267503
Titanium, foil, thickness 0.25 mm, 99.7% trace metals basis
Ti
366994
Titanium, powder, <45 μm avg. part. size, 99.98% trace metals basis
Ti
268496
Titanium, powder, −100 mesh, 99.7% trace metals basis
Ti
348848
Titanium, foil, thickness 0.025 mm, 99.98% trace metals basis
Ti
305812
Titanium, crystalline, 5-10 mm, ≥99.99% trace metals basis (purity exclusive of Na and K content)
Ti
268526
Titanium, sponge, 3-19 mm, 99.5% trace metals basis
Ti
460400
Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis
Ti
460397
Titanium, foil, thickness 0.127 mm, ≥99.99% trace metals basis
Ti
369489
Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis
Ti
267902
Titanium, wire, diam. 0.81 mm, 99.7% trace metals basis
Ti
GF03980932
Titanium, mesh, 150x150mm, nominal aperture 0.19mm, wire diameter 0.23mm, 60x60 wires/inch, open area 20%, twill weave
Ti
267481
Titanium, foil, thickness 0.25 mm, 99.99% trace metals basis
Ti
433667
Titanium, evaporation slug, diam. × L 6.3 mm × 6.3 mm, ≥99.99% trace metals basis
Ti
348856
Titanium, wire, diam. 2.0 mm, 99.99% trace metals basis
Ti
347132
Titanium, rod, diam. 6.35 mm, 99.99% trace metals basis
Ti
266035
Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis
Ti
GF76772391
Titanium, rod, 100mm, diameter 10mm, annealed, 99.6+%
Ti
GF24976525
Titanium, rod, 200mm, diameter 25mm, annealed, 99.6+%
Ti
GF90731505
Titanium, tube, 500mm, outside diameter 0.51mm, inside diameter 0.35mm, wall thickness 0.08mm, annealed, 99.6+%
Ti
GF62738006
Titanium, tube, 500mm, outside diameter 0.81mm, inside diameter 0.61mm, wall thickness 0.1mm, as drawn, 99.6+%
Ti
GF66180034
Titanium, tube, 500mm, outside diameter 1.05mm, inside diameter 0.75mm, wall thickness 0.15mm, annealed, 99.6+%
Ti
GF86399106
Titanium, tube, 500mm, outside diameter 1.6mm, inside diameter 1.2mm, wall thickness 0.2mm, hard, 99.6+%
Ti
GF32919925
Titanium, tube, 500mm, outside diameter 10.3mm, inside diameter 8.7mm, wall thickness 0.8mm, annealed, 99.6+%
Ti
GF64001214
Titanium, tube, 500mm, outside diameter 10mm, inside diameter 8mm, wall thickness 1.0mm, annealed, 99.6+%
Ti
GF71724124
Titanium, tube, 500mm, outside diameter 12.7mm, inside diameter 10.9mm, wall thickness 0.9mm, annealed, 99.6+%
Ti
GF29584187
Titanium, tube, 500mm, outside diameter 2.03mm, inside diameter 1.55mm, wall thickness 0.24mm, annealed, 99.6+%
Ti
GF72193114
Titanium, tube, 500mm, outside diameter 2.03mm, inside diameter 1.63mm, wall thickness 0.2mm, annealed, 99.6+%
Ti
GF26602321
Titanium, tube, 500mm, outside diameter 25.4mm, inside diameter 23.62mm, wall thickness 0.89mm, annealed, 99.6+%
Ti
GF95731960
Titanium, tube, 500mm, outside diameter 3.0mm, inside diameter 1.5mm, wall thickness 0.75mm, annealed, 99.6+%
Ti