EMAIL THIS PAGE TO A FRIEND

Acta biomaterialia

Mobility of the Arg-Gly-Asp ligand on the outermost surface of biomaterials suppresses integrin-mediated mechanotransduction and subsequent cell functions.


PMID 25463493

Abstract

Mechanotransduction in the regulation of cellular responses has been previously studied using elastic hydrogels. Because cells interact only with the surface of biomaterials, we are focusing on the molecular mobility at the outermost surface of biomaterials. In this study, surfaces with the mobile Arg-Gly-Asp-Ser (RGDS) peptide have been constructed. Cell culture substrates were coated with ABA-type block copolymers composed of poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate) segments (A) and a polyrotaxane (PRX) unit with RGDS bound to α-cyclodextrin (B). Adhesion, morphological changes and actin filament formation of human umbilical vein endothelial cells were reduced on the surfaces containing mobile PRX-RGDS in comparison to the immobile RGDS surfaces constructed from random copolymers with RGDS side groups (Prop-andom-RGDS). In the neurite outgrowth assay using rat adrenal pheochromocytoma cells (PC12), only ∼20% of adherent PC12 cells had neurites on PRX-RGDS surfaces, but more than 50% did on the Random-RGDS surface. The beating colony of dimethyl-sulfoxide-treated mouse embryonic carcinoma cells (P19CL6) were found 10 and 14 days after induction on PRX-RGDS and Random-RGDS surfaces, respectively. After 22 days, the beating colony disappeared on PRX-RGDS surfaces, but many colonies remained on Random-RGDS surfaces. These data suggest that the molecular mobility of the cell-binding ligand on the outermost surface of materials effectively suppresses the actin filament formation and differentiation of these functional cell lines, and may be used as a culture substrate for immature stem cells or progenitor cells.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

157260
1-Hydroxybenzotriazole hydrate, wetted with not less than 14 wt. % water, 97%
C6H5N3O · xH2O
711489
1-Hydroxybenzotriazole hydrate, wetted with not less than 20 wt. % water, 97%
C6H5N3O · xH2O
441090
2,2′-Azobis(2-methylpropionitrile), 98%
C8H12N4
755745
2,2′-Azobis(2-methylpropionitrile), recrystallized, 99%
C8H12N4
691275
Acetic anhydride, Lonza quality, ≥99.5% (GC)
C4H6O3
242845
Acetic anhydride, ACS reagent, ≥98.0%
C4H6O3
320102
Acetic anhydride, ReagentPlus®, ≥99%
C4H6O3
539996
Acetic anhydride, 99.5%
C4H6O3
33214
Acetic anhydride, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99% (GC)
C4H6O3
45840
Acetic anhydride
C4H6O3
L095004
Acetic anhydride
C4H6O3
66742
Dichloromethane, Selectophore, ≥99.5%
CH2Cl2
02575
Dichloromethane, analytical standard
CH2Cl2
PHR1553
Dimethylformamide, Pharmaceutical Secondary Standard; Certified Reference Material
C3H7NO
47627
Fmoc-Gly-OH, ≥98.0% (T)
C17H15NO4
64120
Methacryloyl chloride, purum, dist., ≥97.0% (GC), contains ~0.02% 2,6-di-tert-butyl-4-methylphenol as stabilizer
C4H5ClO
523216
Methacryloyl chloride, 97%, contains ~200 ppm monomethyl ether hydroquinone as stabilizer
C4H5ClO
M1550000
Methylene chloride, European Pharmacopoeia (EP) Reference Standard
CH2Cl2
PHR1557
Methylene Chloride, Pharmaceutical Secondary Standard; Certified Reference Material
CH2Cl2
387649
N,N-Diisopropylethylamine, purified by redistillation, 99.5%
C8H19N
496219
N,N-Diisopropylethylamine, 99.5%, biotech. grade
C8H19N
D125806
N,N-Diisopropylethylamine, ReagentPlus®, ≥99%
C8H19N
550043
N,N-Diisopropylethylamine, ≥99%
C8H19N
72438
N,N-Dimethylformamide, analytical standard
C3H7NO
03440
N-Ethyldiisopropylamine, BASF quality, ≥98.0%
C8H19N
03439
N-Ethyldiisopropylamine solution, for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
C8H19N
411027
Piperidine, ≥99.5%, purified by redistillation
C5H11N
571261
Piperidine, biotech. grade, ≥99.5%
C5H11N
80640
Piperidine, puriss. p.a., ≥99.0% (GC/T)
C5H11N
104094
Piperidine, ReagentPlus®, 99%
C5H11N