EMAIL THIS PAGE TO A FRIEND

Journal of biomedical materials research. Part A

Nanocomposite bone scaffolds based on biodegradable polymers and hydroxyapatite.


PMID 25504776

Abstract

In tissue engineering, development of an osteoconductive construct that integrates with host tissue remains a challenge. In this work, the effect of bone-like minerals on maturation of pre-osteoblast cells was investigated using polymer-mineral scaffolds composed of poly(propylene fumarate)-co-poly(caprolactone) (PPF-co-PCL) and nano-sized hydroxyapatite (HA). The HA of varying concentrations was added to an injectable formulation of PPF-co-PCL and the change in thermal and mechanical properties of the scaffolds was evaluated. No change in onset of degradation temperature was observed due to the addition of HA, however compressive and tensile moduli of copolymer changed significantly when HA amounts were increased in composite formulation. The change in mechanical properties of copolymer was found to correlate well to HA concentration in the constructs. Electron microscopy revealed mineral nucleation and a change in surface morphology and the presence of calcium and phosphate on surfaces was confirmed using energy dispersive X-ray analysis. To characterize the effect of mineral on attachment and maturation of pre-osteoblasts, W20-17 cells were seeded on HA/copolymer composites. We demonstrated that cells attached more to the surface of HA containing copolymers and their proliferation rate was significantly increased. Thus, these findings suggest that HA/PPF-co-PCL composite scaffolds are capable of inducing maturation of pre-osteoblasts and have the potential for use as scaffold in bone tissue engineering.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

263230
Germanium, chips, 99.999% trace metals basis
Ge
203343
Germanium, chips, 99.999% trace metals basis
Ge
327395
Germanium, powder, −100 mesh, ≥99.999% trace metals basis
Ge
203351
Germanium, powder, −100 mesh, ≥99.99% trace metals basis
Ge
GF58081451
Germanium, sheet, 50x50mm, thickness 3.0mm, polycrystalline, 99.999%
Ge
GF99720647
Germanium, sheet, 50x50mm, thickness 1.0mm, polycrystalline, 99.999%
Ge
GF31896366
Germanium, sheet, 7x24mm, thickness 1.0mm, polycrystalline, 99.999%
Ge
GF46650090
Germanium, disks, 15mm, thickness 1.0mm, polycrystalline, 100%
Ge
GF90754858
Germanium, disks, 20mm, thickness 1.0mm, single crystal, 100%
Ge
GF77697680
Germanium, microfoil, 25x25mm, thinness 0.25μm, specific density 166.3μg/cm2, 6 micron aluminum permanent support, 100%
Ge
GF58880739
Germanium, microfoil, 25x25mm, thinness 0.5μm, specific density 333μg/cm2, 6 micron aluminum permanent support, 100%
Ge
GF12210624
Germanium, microfoil, 50x50mm, thinness 0.25μm, specific density 166.3μg/cm2, 6 micron aluminum permanent support, 100%
Ge
GF57527219
Germanium, rod, 25mm, diameter 2.0mm, polycrystalline, n-type, 99.999%
Ge
GF65029417
Germanium, rod, 25mm, diameter 5mm, polycrystalline, n-type, 99.999%
Ge
GF14446346
Germanium, rod, 50mm, diameter 5mm, polycrystalline, n-type, 99.999%
Ge
GF04605780
Germanium, rod, 6mm, diameter 6.0mm, single crystal, 100%
Ge
GF09210417
Germanium, sheet, 10x10mm, thickness 0.10mm, polycrystalline, 99.999%
Ge
GF00633664
Germanium, sheet, 10x10mm, thickness 0.25mm, polycrystalline, 99.999%
Ge
GF09629859
Germanium, sheet, 10x10mm, thickness 0.5mm, single crystal, -111, 100%
Ge
GF45727241
Germanium, sheet, 10x10mm, thickness 0.6mm, single crystal, -111, 100%
Ge
GF44621580
Germanium, sheet, 25x25mm, thickness 1.0mm, polycrystalline, 99.999%
Ge
GF44356978
Germanium, sheet, 25x25mm, thickness 1.0mm, single crystal, 99.999%
Ge
GF20558022
Germanium, sheet, 25x25mm, thickness 3.0mm, polycrystalline, 99.999%
Ge
GF49319815
Germanium, sheet, 4x4mm, thickness 0.05mm, polycrystalline, 99.999%
Ge
GF73619107
Germanium, sheet, 50x50mm, thickness 0.5mm, single crystal, 99.999%
Ge
GF37208369
Germanium, sheet, 6x6mm, thickness 1.0mm, polycrystalline, 99.999%
Ge