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Anticancer research

Elimination of Ras Suppressor-1 from hepatocellular carcinoma cells hinders their in vitro metastatic properties.


PMID 25750304

Abstract

Extracellular matrix (ECM) is of great significance for homeostasis in the liver. In fact, one of the stages leading to hepatocellular carcinoma (HCC) includes accumulation of excess ECM. Ras Suppressor-1 (RSU-1) is localized in the cell-ECM adhesions but its role in HCC is unexplored. We investigated the expression and role of RSU-1 in two HCC cell lines that differ in aggressiveness; non-invasive Alexander cells and highly invasive HepG2 cells. Our results showed that RSU-1 expression is elevated in HepG2 cells both at the mRNA and protein level, while its silencing leads to increased cell proliferation in both cell lines. Interestingly, RSU-1 depletion from highly invasive HepG2 cells reduces cell adhesion and invasion. This is the first study to provide in vitro evidence for the involvement of RSU-1 in HCC cell invasive behavior.