International journal of pharmaceutics

In vitro and in vivo evaluation of APRPG-modified angiogenic vessel targeting micelles for anticancer therapy.

PMID 25840274


The study was aimed to evaluate the antitumor potential of the Ala-Pro-Arg-Pro-Gly (APRPG)-modified angiogenic vessel targeting drug delivery system using paclitaxel (PTX) as a model drug. In this study, an angiogenesis homing peptide APRPG was conjugated to the amphipathic copolymer PLGA-PEG and the synthesized copolymer APRPG-PEG-PLGA was used to prepare PTX encapsulated micelles (APRPG-PEG-Mic). The micelles were uniform spherical and exhibited a unimodal particle size distribution and a slight negative zeta-potential. The in vitro drug release result demonstrated a significant sustained release property of APRPG-PEG-Mic. Compared to Taxol(®) and Cont-PEG-Mic, APRPG-PEG-Mic showed a stronger cytotoxicity against two cancerous cell lines. In the cell uptake studies, the APRPG-modified micelles enhanced intracellular fluorescent intensity in EA.hy926 cells. The biodistribution study revealed the accumulation of APRPG-PEG-Mic in tumor tissues as a result of passive accumulation and active targeting. In comparison with Taxol(®) and Cont-PEG-Mic, APRPG-PEG-Mic reduced the tumor volume more significantly and prolonged the survival time of tumor-bearing mice, indicating a higher antitumor efficacy and lower systematic side effects of APRPG-PEG-Mic. The results indicated that APRPG-modified micelles could be an efficient target-delivery method to angiogenic vessels and a highly promising therapeutic system in anticancer therapy.