EMAIL THIS PAGE TO A FRIEND

PloS one

Perilipin1 deficiency in whole body or bone marrow-derived cells attenuates lesions in atherosclerosis-prone mice.


PMID 25855981

Abstract

The objective of this study is to determine the role of perilipin 1 (Plin1) in whole body or bone marrow-derived cells on atherogenesis. Accumulated evidence have indicated the role of Plin1 in atherosclerosis, however, these findings are controversial. In this study, we showed that Plin1 was assembled and colocalized with CD68 in macrophages in atherosclerotic plaques of ApoE-/- mice. We further found 39% reduction of plaque size in the aortic roots of Plin1 and ApoE double knockout (Plin1-/-ApoE-/-) females compared with ApoE-/- female littermates. In order to verify whether this reduction was macrophage-specific, the bone marrow cells from wild-type or Plin1 deficient mice (Plin1-/-) were transplanted into LDL receptor deficient mice (LDLR-/-). Mice receiving Plin1-/- bone marrow cells showed also 49% reduction in aortic atherosclerotic lesions compared with LDLR-/- mice received wild-type bone marrow cells. In vitro experiments showed that Plin1-/- macrophages had decreased protein expression of CD36 translocase and an enhanced cholesterol ester hydrolysis upon aggregated-LDL loading, with unaltered expression of many other regulators of cholesterol metabolism, such as cellular lipases, and Plin2 and 3. Given the fundamental role of Plin1 in protecting LD lipids from lipase hydrolysis, it is reasonably speculated that the assembly of Plin1 in microphages might function to reduce lipolysis and hence increase lipid retention in ApoE-/- plaques, but this pro-atherosclerotic property would be abrogated on inactivation of Plin1. Plin1 deficiency in bone marrow-derived cells may be responsible for reduced atherosclerotic lesions in the mice.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1401062
Anti-CD68 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB4501762
Anti-HSL antibody produced in rabbit, affinity isolated antibody
SAB4501763
Anti-HSL antibody produced in rabbit, affinity isolated antibody
SAB2900275
Anti-LIPE antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
P1998 Anti-Perilipin A antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2500775
Anti-Perilipin, C-Terminal antibody produced in goat, affinity isolated antibody, buffered aqueous solution
AV42057
Anti-PLIN antibody produced in rabbit, IgG fraction of antiserum
HPA024299
Anti-PLIN1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1411126
Anti-PNPLA2 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB1402677 Monoclonal Anti-ABCA1 antibody produced in mouse, clone 1H4, ascites fluid
AMAB90873
Monoclonal Anti-CD68 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL1338, purified immunoglobulin, buffered aqueous glycerol solution
AMAB90874
Monoclonal Anti-CD68 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL1346, purified immunoglobulin, buffered aqueous glycerol solution
SAB1402313
Monoclonal Anti-PLIN antibody produced in mouse, clone 4G1, purified immunoglobulin, buffered aqueous solution
PRS3965
Anti-Lipe antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution