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Bioprocess and biosystems engineering

Effective inactivation of Candida albicans biofilms by using supercritical carbon dioxide.


PMID 26109343

Abstract

Present sterilization methods for biofilms in medical devices have limitations. Therefore, an alternative sterilization method using supercritical carbon dioxide (SC-CO2) was tested on Candida albicans biofilms. The effect of varying pressure, temperature, and treatment time on the inactivation of C. albicans spores in suspensions and in biofilms was examined. The parameters such as treatment time, pressure, and temperature that led to the complete inactivation of C. albicans biofilms ranged 5-20xa0min, 100-200xa0bar, and 35-45xa0°C, respectively. Notably, treatment of SC-CO2 at either 100xa0bar and 40xa0°C or 200xa0bar and 30xa0°C induced complete inactivation of spores within 5xa0min. Furthermore, it was found that wet biofilms (0.4xa0%, w/w) had higher sensitivity to SC-CO2 than dried biofilms. Finally, spore inactivation was confirmed by confocal laser scanning microscopy. In this study, the use of a low-temperature SC-CO2 sterilization method was proven to be effective in fungal biofilm inactivation, and the moisture content of biofilms was revealed to be the key factor for biofilm inactivation.