Chinese medicine

Crude triterpenoid saponins from Ilex latifolia (Da Ye Dong Qing) ameliorate lipid accumulation by inhibiting SREBP expression via activation of AMPK in a non-alcoholic fatty liver disease model.

PMID 26300958


Ilex latifolia Thunb. (Da Ye Dong Qing) is used for weight loss and for its antidiabetic effects. This study aims to investigate the beneficial effects and potential mechanisms of action of crude triterpenoid saponins (CTS) from I. latifolia in a mouse model of high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD). Male C57BL/6 mice (nxa0=xa050), were arbitrarily divided into five groups (nxa0=xa010 in each group): a control group, HFD group, simvastatin group (10xa0mg/kg/day), and two CTS treatment groups (100 and 200xa0mg/kg/day). All mice except those in the control group were fed an HFD for 4xa0weeks. Animals in the treatment groups were orally administered simvastatin or CTS for 8xa0weeks. Oral glucose tolerance tests and insulin tolerance tests were performed. At the end of treatment, plasma lipid levels, and oxidative parameters in the liver were measured using commercial test kits. Western blotting was used to evaluate whether CTS induced AMP-activated protein kinase (AMPK) and acetyl CoA carboxylase activation, and the expression of transcription factors and their target genes was evaluated in a quantitative PCR assay. Compared with the HFD group, the CTS (200xa0mg/kg/day) treatment group showed significantly decreased plasma lipid parameters (Pxa0