Molecular oral microbiology

Comparison of inherently essential genes of Porphyromonas gingivalis identified in two transposon-sequencing libraries.

PMID 26358096


Porphyromonas gingivalis is a Gram-negative anaerobe and keystone periodontal pathogen. Axa0mariner transposon insertion mutant library has recently been used to define 463 genes as putatively essential for the inxa0vitro growth of P.xa0gingivalis ATCC 33277 in planktonic culture (Library 1). We have independently generated a transposon insertion mutant library (Library 2) for the same P.xa0gingivalis strain and herein compare genes that are putatively essential for inxa0vitro growth in complex media, as defined by both libraries. In all, 281 genes (61%) identified by Library 1 were common to Library 2. Many of these common genes are involved in fundamentally important metabolic pathways, notably pyrimidine cycling as well as lipopolysaccharide, peptidoglycan, pantothenate and coenzyme Axa0biosynthesis, and nicotinate and nicotinamide metabolism. Also in common are genes encoding heat-shock protein homologues, sigma factors, enzymes with proteolytic activity, and the majority of sec-related protein export genes. In addition to facilitating a better understanding of critical physiological processes, transposon-sequencing technology has the potential to identify novel strategies for the control of P.xa0gingivalis infections. Those genes defined as essential by two independently generated TnSeq mutant libraries are likely to represent particularly attractive therapeutic targets.

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(−)-Nicotine, PESTANAL®, analytical standard