EMAIL THIS PAGE TO A FRIEND

Nature methods

A strategy for dissecting the architectures of native macromolecular assemblies.


PMID 26436480

Abstract

It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.