EMAIL THIS PAGE TO A FRIEND

Analytica chimica acta

A novel approach for LC-MS/MS-based chiral metabolomics fingerprinting and chiral metabolomics extraction using a pair of enantiomers of chiral derivatization reagents.


PMID 26526912

Abstract

Chiral metabolites are found in a wide variety of living organisms and some of them are understood to be physiologically active compounds and biomarkers. However, the overall analysis of chiral metabolomics is quite difficult due to the high number of metabolites, the significant diversity in their physicochemical properties, and concentration range from metabolite-to-metabolite. To solve this difficulty, we developed a novel approach for chiral metabolomics fingerprinting and chiral metabolomics extraction, which is based on the labeling of a pair of enantiomers of chiral derivatization reagents (i.e., DMT-(S,R)-Pro-OSu and DMT-3(S,R)-Apy) and precursor ion scan chromatography of the derivatives. The multivariate statistics is also required for this strategy. The proposed procedures were evaluated by the detection of a diagnostic marker (i.e., d-lactic acid) using the saliva of diabetic patients. This method was used for the determination of biomarker candidates of chiral amines and carboxyls in Alzheimer's disease (AD) brain homogenates. As the results, l-phenylalanine (L-Phe) and l-lactic acid (L-LA) were identified as the decreased and increased biomarker candidates in the AD brain, respectively. Therefore, the proposed approach seems to be helpful for the determination of non-target chiral metabolomics possessing amines and carboxyls.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

284505
1,2-Dichloroethane, anhydrous, 99.8%
C2H4Cl2
D23602
1,3-Diaminopropane, ≥99%
C3H10N2
721832
3-(Boc-amino)pyrrolidine, 95%
C9H18N2O2
107700
4-(Dimethylamino)pyridine, ReagentPlus®, ≥99%
C7H10N2
714844
4-(Dimethylamino)pyridine solution, 0.5 M in THF
C7H10N2
700169
4-(Dimethylamino)pyridine, ChemDose tablets, Loading: 0.04 mmol tablet.
C7H10N2
733342
9-Fluorenyl methacrylate, 97%
C17H14O2
M31104
α-Methylbenzylamine, 99%
C8H11N
33220
Cadaverine dihydrochloride, ≥99.0% (AT)
C5H14N2 · 2HCl
C8561
Cadaverine dihydrochloride, ~98%
C5H14N2 · 2HCl
858919
D-Proline, ReagentPlus®, ≥99%
C5H9NO2
94640
DL-Valine, ReagentPlus®, ≥99.0% (NT)
C5H11NO2
W344400
DL-Valine, ≥97%
C5H11NO2
W248703
Formic acid, ≥95%, FCC, FG
CH2O2
ARK2164
Fumaric acid, PharmaGrade, USP/NF, Manufactured under appropriate GMP controls for pharma or biopharmaceutical production.
C4H4O4
F8509
Fumaric acid, BioReagent, suitable for cell culture
C4H4O4
W248800
Fumaric acid, FCC, FG
C4H4O4
A5835
γ-Aminobutyric acid, BioXtra, ≥99%
C4H9NO2
A2129
γ-Aminobutyric acid, ≥99%
C4H9NO2
A6251
N-Acetyl-DL-tryptophan
C13H14N2O3
ARK2160C
N-Acetyl-DL-tryptophan, PharmaGrade, Manufactured under appropriate GMP controls for pharma or biopharmaceutical production., EP
C13H14N2O3
130672
N-Hydroxysuccinimide, 98%
C4H5NO3
P7505
Putrescine dihydrochloride, ≥98% (TLC)
C4H12N2 · 2HCl
P5780
Putrescine dihydrochloride, powder, BioReagent, suitable for cell culture
C4H12N2 · 2HCl
P6024
Putrescine dihydrochloride, γ-irradiated, lyophilized powder, BioXtra, suitable for cell culture
C4H12N2 · 2HCl
S7672
Sarcosine, BioXtra
C3H7NO2
131776
Sarcosine, 98%
C3H7NO2
S3674
Succinic acid, BioXtra, ≥99.0%
C4H6O4
S9512
Succinic acid, BioReagent, suitable for cell culture, suitable for insect cell culture
C4H6O4
398055
Succinic acid, ACS reagent, ≥99.0%
C4H6O4