Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Amphetamine and derivatives in natural weight loss pills and dietary supplements by capillary electrophoresis-tandem mass spectrometry.

PMID 27776329


A capillary electrophoresis-tandem mass spectrometry (CE-MS/MS) method for amphetamine (AM), phentermine (PTM), methamphetamine (MAM), methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), and methylenedioxyethylamphetamine (MDEA) in commercial samples of homeopathic and phytotherapic medicines and dietary supplements is presented. The samples were submitted to a modified QuEChERS extraction procedure (at apparent pH 13) followed by electrophoretic separation in 0.1molL(-1) formic acid electrolyte (pH 2.4) and detection by ESI-MS/MS. A polyvinyl alcohol coated capillary was employed to prevent the adsorption of the analytes to the capillary wall. The limits of detection and quantitation were from 0.02 to 0.06μgL(-1) and from 0.06 to 0.21μgL(-1), respectively, with recovery ranging from 85 to 123% and the standard deviations were not greater than 6.1%. In addition, the separation occurs in less than six minutes.