Head & neck

p16(INK4a) : A surrogate marker of high-risk human papillomavirus infection in squamous cell carcinoma of the nasal vestibule.

PMID 28371015


The purpose of this study was to analyze the role of p16(INK4a) and the prevalence of human papillomavirus (HPV) in squamous cell carcinoma (SCC) of the nasal vestibule. Patients diagnosed from 1995 to 2014 were included in this study. Assessment of p16(INK4a) and HPV-DNA polymerase chain reaction (PCR) was performed and analyzed with respect to baseline, clinicopathological, and outcome parameters. The p16(INK4a) positivity was defined as unequivocal nuclear and cytoplasmic staining of ≥70% of the cells, whereas 50%-69% was considered to be a "borderline" result. There were 46 patients with SCCs of the nasal vestibule, of whom 31 (67.4%) were available for p16(INK4a) and 30 (65.2%) for analysis of HPV. Expression of p16(INK4a) was present in 19.4% and showed coincidence with high-risk HPV (P < .001). Neither p16(INK4a) nor HPV-DNA had significant impact on outcome. Significant immunoreactivity for p16(INK4a) was present in about one-fifth of the samples and figured as a surrogate marker of high-risk HPV infection. There was no influence on outcome.