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Journal of bioscience and bioengineering

Size- and time-dependent growth properties of human induced pluripotent stem cells in the culture of single aggregate.


PMID 28601606

Abstract

Aggregate culture of human induced pluripotent stem cells (hiPSCs) is a promising method to obtain high number of cells for cell therapy applications. This study quantitatively evaluated the effects of initial cell number and culture time on the growth of hiPSCs in the culture of single aggregate. Small size aggregates ((1.1 ± 0.4) × 10