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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

The step-wise framework to design a chromatography-based hydrophobicity assay for viral particles.


PMID 28818800

Abstract

A high-salt, hydrophobic interaction chromatography (HIC) method was developed to measure the relative hydrophobicity of a diverse set of solutes. Through the careful control of buffer pH and salt concentration, this assay was then used to ascertain for the first time the relative hydrophobicity values of three different bacteriophage, four mammalian viruses, and a range of biotech medicinal proteins as benchmarked to protein standards previously characterized for hydrophobicity.