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The Analyst

Branched polyethyleneimine-assisted boronic acid-functionalized magnetic nanoparticles for the selective enrichment of trace glycoproteins.


PMID 29094730

Abstract

Boronate affinity materials, as efficient sorbents for extraction, separation and enrichment of glycoproteins, have attracted more and more attention in recent years. However, conventional boronate affinity materials suffer from low binding affinity due to the use of single boronic acids, which makes the extraction of glycoproteins in trace concentration rather difficult or impossible. Here we present a type of high boronate avidity material, polyethyleneimine (PEI)-assisted boronic acid-functionalized magnetic nanoparticles (MNPs). Branched PEI was used as a scaffold to amplify the number of boronic acid moieties, while 2,4-difluoro-3-formyl-phenylboronic acid (DFFPBA), exhibiting high affinity and excellent water solubility toward cis-diol-containing compounds, was employed as an affinity ligand. Due to the PEI-assisted synergistic multivalent binding, the boronate avidity MNPs exhibited a high binding affinity toward glycoproteins with dissociation constants of 10

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597406
2,4-Difluoro-3-formylphenylboronic acid
C7H5BF2O3