EMAIL THIS PAGE TO A FRIEND

Nature communications

A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion.


PMID 29950589

Abstract

Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

250910
2-Ethoxyphenol, 98%
C8H10O2
M13203
3-Methoxycatechol, 99%
C7H8O3