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Transactions of the Royal Society of Tropical Medicine and Hygiene

Aminomethyl coumarin acetic acid and fluorescein isothiocyanate in detection of leishmanial antibodies: a comparative study.


PMID 3151411

Abstract

Fluorescein isothiocyanate incorporated in the immunofluorescent antibody test system used for serodiagnosis of leishmanial and other infections suffers certain inherent disadvantages. Aminomethyl coumarin acetic acid, a new fluorescent label for protein, yields better results regarding non-specific background staining and stability, with slower photodecomposition under ultra-violet light when used for detection of leishmanial antibodies. The new labelling agent emits a yellow/green colour with an excitation filter of 450-490 nm and a barrier filter of 520 nm.

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