EMAIL THIS PAGE TO A FRIEND

Biochimica et biophysica acta

Molecular cloning of a human gastric lipase and expression of the enzyme in yeast.


PMID 3304425

Abstract

The molecular cloning of a cDNA coding for human gastric lipase and its expression in yeast is described. A lipase present in human gastric aspirates was purified and its N-terminal amino-acid sequence was determined. This was found to be homologous with the N-terminal sequence of rat lingual lipase. A cDNA library was constructed from mRNA isolated from human stomach tissue and probed with cloned rat lingual lipase DNA. One clone, pGL17, consisting of approximately 1450 base-pairs, contained the entire coding sequence for a human gastric lipase. The amino-acid sequence from the isolated protein and the DNA sequence obtained from the cloned gene indicated that human gastric lipase consists of a 379 amino acid polypeptide with an unglycosylated Mr of 43,162. Human gastric lipase and rat lingual lipase amino-acid sequences were closely homologous but were unrelated to porcine pancreatic lipase apart from a 6 amino-acid sequence around the essential Ser-152 of porcine pancreatic lipase. A yeast expression plasmid containing the phosphoglycerate kinase promoter and terminator sequences together with the human gastric lipase gene was constructed. Yeast transformed with this vector synthesised the lipolytically active enzyme.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

62287 Lipase A Candida antarctica, recombinant from Aspergillus oryzae, powder, beige, ~2 U/mg
L4777
Lipase acrylic resin, ≥5,000 U/g, recombinant, expressed in Aspergillus niger
62288 Lipase B Candida antarctica, recombinant from Aspergillus oryzae, powder, beige, ~9 U/mg
62301 Lipase from Aspergillus niger, powder (fine), ~200 U/g
L4277 Lipase from Aspergillus oryzae, ≥20,000 U/g
62285 Lipase from Aspergillus oryzae, lyophilized, powder, white, ~50 U/mg
75577 Lipase from Burkholderia sp., lyophilized powder, slightly beige, ~12 U/mg
02569 Lipase from Candida antarctica, lyophilized, powder, beige, ~0.3 U/mg
62302 Lipase from Candida rugosa, lyophilized, powder (fine), 15-25 U/mg
L1754 Lipase from Candida rugosa, Type VII, ≥700 unit/mg solid
62316 Lipase from Candida rugosa, powder, yellow-brown, ≥2 U/mg
L8525 Lipase from Candida rugosa, lyophilized powder, ≥40,000 units/mg protein
L3170
Lipase from Candida sp., recombinant, expressed in Aspergillus niger
L8906 Lipase from Mucor javanicus, lyophilized powder, ≥300 units/mg solid (using olive oil)
L9031 Lipase from Mucor miehei, lyophilized powder, ≥4,000 units/mg solid (using olive oil)
62298 Lipase from Mucor miehei, powder, slightly brown, ~1 U/mg
L0382 Lipase from porcine pancreas, Type VI-S, ≥20,000 units/mg protein, lyophilized powder
62309 Lipase from Pseudomonas cepacia, powder, light beige, ≥30 U/mg
28602 Lipase from Pseudomonas fluorescens, powder, slightly beige, ≥160 U/mg
L9518
Lipase from Pseudomonas sp., Type XIII, lyophilized powder, ≥15 units/mg solid
62310 Lipase from Rhizopus niveus, powder (fine), ≥1.5 U/mg
L3001 Lipase from wheat germ, Type I, lyophilized powder, 5-15 units/mg protein
62306 Lipase from wheat germ, ~0.1 U/mg
73940 Lipase immobilized from Candida antarctica, beads, slightly brown, >2 U/mg