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Clinical chemistry

Chemiluminescence enzyme immunoassay for thyroxin with use of glucose oxidase and a bis(2,4,6-trichlorophenyl)oxalate-fluorescent dye system.


PMID 3882274

Abstract

In this chemiluminescence enzyme immunoassay for thyroxin, glucose oxidase (EC 1.1.3.4) is the enzyme label and the bound and free fractions are separated by the double-antibody solid-phase technique. In the assay of enzyme activity, hydrogen peroxide generated from glucose substrate is measured by its chemiluminescence reaction with bis(2,4,6-trichlorophenyl)oxalate and 8-anilino-1-naphthalene-sulfonic acid. The measurable range of thyroxin is 2.5 to 200 micrograms/L. The coefficients of variation for thyroxin concentrations of 43.2 and 12.8 micrograms/L were 7.0% and 8.6% (intra-assay), and 6.4% and 12.3% (interassay), respectively. The proposed method is applicable to large-scale preliminary screening of neonates for congenital hypothyroidism, with samples consisting of dried blood spotted on filter paper.

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