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Clinica chimica acta; international journal of clinical chemistry

Fluorometric measurement of glycosylated albumin in human serum.


PMID 4028431

Abstract

A simple and rapid fluorometric assay of glycosylated albumin was developed using dansylated phenyl boronic acid (N-(5-dimethyl amino-1-naphthalene sulfonyl)-3-aminobenzene boronic acid). The emission spectrum of the reagent changes upon binding to cis-diols present on glycosylated albumin in serum, allowing quantitation of glycosylated albumin. Results from the new procedure correlated well with those from commercially prepared affinity chromatography (r = 0.85).

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30423
3-(Dansylamino)phenylboronic acid, BioReagent, suitable for fluorescence, ≥98.0% (HPLC)
C18H19BN2O4S