EMAIL THIS PAGE TO A FRIEND

The Histochemical journal

A direct-colouring, metal precipitation method for the demonstration of arylsulphatases A and B.


PMID 6145687

Abstract

A new, direct-colouring, metal precipitation method for the light microscopical demonstration of arylsulphatases A and B is described. It is based on the reducing capacity of nitrocatechol liberated by arylsulphatases from p-nitrocatechol sulphate. The reaction is carried out in Karnovsky-Roots' copper ferricyanide incubation mixture at pH 5.0 or 5.5; the nitrocatechol liberated reduces ferricyanide to ferrocyanide, which in turn forms a brown precipitate with copper that indicates enzyme activity. Enzyme activity was localized in cytoplasmic particles compatible with a lysosomal localization of the enzyme. The histochemical reaction was inhibited by phosphate, which has been shown to inhibit arylsulphatases A and B in biochemical determinations. The method described is technically simple, and sections can be mounted in synthetic resin after dehydration.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

N7251
4-Nitrocatechol sulfate dipotassium salt, crystalline
C6H3K2NO7S